Chemokine receptors are fundamentally important for leukocyte migration during inflammation and at homeostasis by detection of extracellular chemokine signals. Furthermore, these receptors are increasingly used as important surface markers of functional subset of T cells. In this presentation I will describe the work of the chemokine biology laboratory at the University of Adelaide and how we are utilising flow cytometry and FACS sorting in a variety of experimental settings. At the Molecular & Biomedical Sciences flow cytometry facility (utilising BD Fortessa X20 and BD FACSAriaIIImu instruments) we routinely perform various multicolour panels involving intracellular cytokines and transcription factors to identify cell populations of interest and also utilise cytokine-capture technology to purify novel subsets of murine T cells. In particular, I will describe our work on various subsets of T cells that can be identified using chemokine receptor profiling in mouse models of autoimmunity, viral infection and cancer. These include distinct subsets of Th17 cells, GM-CSF-producing CD4+ T cells, gamma-delta-17 cells (before and after activation), novel subsets of CD8+ T cells and type-1 regulatory T cells. The new insights into these novel cell types and their roles in the immune response that is revealed using flow cytometry-based technology is increasing our basic understanding of the workings of the adaptive immune system.