Proliferation and differentiation programs throughout haematopoiesis involve multifactorial coordination, which if perturbed can cause pathogenic alteration of particular lineages, including leukaemic development. Mouse models have been used extensively to characterise many genetic alterations that lead to leukaemia induction. When modelling the effects of a mutation, or an exogenous treatment on the haemopoiesis system in mouse models, detailed analysis using multi-colour flow cytometry is useful to assess changes in the progenitor cell compartment from bone marrow. For this a defined combination of lineage differentiation markers specific for mouse is required. Although Weissman et al. and Pronk et al. have reported multi-colour-flow cytometry for the analysis of haemopoietic stem and progenitor cells (HSPCs), and committed myeloid progenitor (CMPs) respectively, our goal was to expand and combine these existing panels for comprehensive leukocyte phenotyping using eleven-colours. We will present the details of our method which allows detection of HSPCs and lineage progenitors in a single tube. This panel can be used for mouse bone marrow and spleen analysis in mouse models of haemopoiesis and leukaemia, and also provides a tool for investigating haemopoiesis responses to drug treatments.