In recent years several instruments have been developed that challenge the one fluorochrome one detector paradigm. By examining the full spectral signature of a number of reference fluorochromes the contribution of each of these dyes in a mixed sample can be determined. This approach can enable dyes which share very similar maximum emission wavelengths to be distinguished from one another.
This workshop strives to explore the abilities of one such instrument, the Aurora™ produced by Cytek Biosciences Inc. Several of these instruments are in operation in Australia and New Zealand. What are the advantages or shortfalls of this platform? How does it compare to conventional cytometers in terms of experimental design, sensitivity, ease of use and robustness?
The presenters will share their experiences on the recently released two laser version of the Aurora™ the Northern Lights™ and the three laser and 4 laser Aurora™ systems. Some examples of experiments will be shown which would have been challenging or impossible on conventional platforms.