Over the in the last few years technologies that allow low cost, high throughput single cell genomic analysis have emerged and have successfully been leveraged in the understanding of both normal function and disease states. Whilst flow cytometry is still the gold standard cellular characterisation tool, the rise of these novel single cell-omics technologies have, in some circles, been seen to be directly challenge the relevance of flow cytometry.
By discussing the technical aspects of technologies such as DropSeq, Chromium 10x, Rhapsody, CiteSeq as well as plate based protocols such as MARSSeq/ CellSeq/SmartSeq and the sequencing considerations that are used with these techniques alongside advanced cytometry techniques such as high dimensional fluorescence (Symphony a5) and Mass Cytometry (CYTOF) we hope to open a dialogue to discuss how genomic cytometry (the practice of measuring cells using traditional genomic technologies) can complement and expand more traditional approaches of cytometry.