The emergence of high-throughput single-cell transcriptomic analyses has revealed an immensely rich complexity of cell types and interactions across a range of disease states and biological systems. Whilst the cytometry world is embracing these new technologies the cytometry SRL faces many challenges with their implementation. These challenges are predominantly around the new field of Genomic Cytometry. Genomic Cytometry involves measuring cells (cytometry) using techniques more often found in the genomics core, notably sequencing and forms the next frontier for the cytometry SRL.
Here we discuss some of the novel technologies at the interface of cytometry and genomics and take a genomics view on how the cytometry SRL can assist in integrating newly emerging Genomic Cytometry workflows. By discussing key considerations for genomics workflow such as laboratory setup, reducing and detecting RNase contamination, a general introduction to sequencing as well as sorting tips when working at the single cell and low cell input levels we hope to shed light on how understanding key genomic concepts will help ensure the cytometry SRL is equipped to deal with the novel frontier that is genomic cytometry.